bblkargonaut

joined 1 year ago
[–] bblkargonaut 32 points 4 months ago (11 children)

The problem isnt woke, the problem is CW level writing. I've watched every episode of star Trek up to season 2 of Picard and the writers of discovery not understanding the speed of light, and it feel like the writers are just in qualified for their positions. It's so bad.

The writing for all the previous shows all had their highs and lows, but characters acted within their characterization and had genuine motivations. Picard season 1 was a rip off of mass effect and old man's war, with the facade Picard shoe horned in. Season 2 you have him chummy with a cold blooded murderer who faced no consequences.

I liked discovery for the first few seasons, but the plot holes, inconsistencies, and poor characterizations, left me feeling like watching the show was more habit or chore. I also never really felt like I got to know characters because their actions are so off the wall that you don't figure out the algorithms of their personality and motivations.

I honestly feel like lower decks has been the best Star Trek made in this new era, but I haven't seen brave new worlds yet or Picard season 3. I just needed a break after Picard season 2.

[–] bblkargonaut 30 points 5 months ago (2 children)

Wait until they learn the Spanish word for black, or worse Latin.

[–] bblkargonaut 11 points 5 months ago (1 children)

Someone in my group chat poster the picture and I can't unsee it. It's horrible.

[–] bblkargonaut 2 points 5 months ago

I played through the entire story, just because of the games theme song. Casey Edwards was really cooking.

[–] bblkargonaut 3 points 5 months ago (1 children)

My alignment is neutral good

[–] bblkargonaut 2 points 5 months ago

Im the one that was paying for Netflix for my family, but the password crack down motivated me to learn how to build a server and go full arrs. They had a good thing going, but now that $26 a month will be used to buy hdds.

[–] bblkargonaut 20 points 5 months ago

I just wanted to see Luke in the role of obi wan or Yoda after decades of Jedi training be a hero and pass on his legacy by training the next generation. But I would have been ok with one heroic lightsaber battle, and a reunion with han, chewy and Leia.

Watching the last Jedi and seeing someone who tossed his blade away because he saw the good in essentially "Space Hitler" try to kill his own nephew because he was having a nightmare so out of character. Then having him overdose on the force and die like a chump, broke me. I left the theater in silence. The last Jedi is also the only star wars movie with out a light saber fight. No blades ever touched.

[–] bblkargonaut 10 points 5 months ago (3 children)

A partial solution is to remove nimby laws that prevent accessory dwelling units, duplexes, and medium and high density housing. Then you use the tax laws to heavily punish corporate ownership of low and medium density housing, and make it progressively more expensive for mom and pop landlords after a reasonable number of properties.

Hopefully this would lower the cost for single family housing, while increasing supply and variety of rentals. But somehow you'd have to prevent end stage capitalist collusion from fixing prices so competition could actually work to drive down prices.

[–] bblkargonaut 3 points 6 months ago (1 children)

Lol yeh but it's partially funded by money saved from canceled subscriptions.

[–] bblkargonaut 3 points 6 months ago (3 children)

I'm a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it's a slippery slope.

[–] bblkargonaut 2 points 6 months ago

Pretty good description. Few extra points. -you don't need to don't need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.

For what is possibly wrong with the top gel.

  • the wavy line in some bands indicates uneven current, this can be due to sample composition before loading the gel, like having cellular debris along with your DNA or a mismatch between the buffer used to make the gel and the running buffer.

-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you'll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it's not pushed in to gel evenly, or it was run to fast.

Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don't know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.

[–] bblkargonaut 2 points 6 months ago

Most definitely and probably be missing some limb by now.

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