this post was submitted on 17 Jun 2023
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Mycology
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I went and skimmed through that whole series, and then I found another video where he discusses the problems of going spore to grain, and even assuming I did get some viable mycelium on these plates, I'm thinking it's going to be nearly impossible to select them properly. I suppose I could just rinse and repeat into new plates, but then I won't know if it's a viable diploid/dikaryon or not.
Yet, the video I initially watched https://www.youtube.com/watch?v=tYK0hLeanVc demonstrated just grabbing some spores with a sterile blade and wiping that across agar. So maybe it is a reasonable method? One thing's for sure, there's a lot of colonies along the inoculation line, and it's already probably too late to start isolating like you would with the method demonstrated by Fresh From the Farm.