Gwindolyn from DS1 was a ladyboy, he also made an appearance in DS3, although has being vored by Aldrich.
And then Miquella from Elden Ring is a ladyboy that loves radahn, a big beefcake looking dude.
I’m pretty sure there was a ladyboy in DS2, although now I’ll be honest that I only played through that once.
Ah, the trauma of every Bethesda RPG player.
There are game studios out there that don’t release broken garbage that needs the player to walk on eggshells, backup saves, and do arcane console commands to make the game playable.